Distinguishing cells from empty droplets in droplet-based single-cell RNA sequencing data
Aaron T. L. Lun, Samantha J. Riesenfeld, Tallulah S. Andrews, The Phuong Dao, Tomás Gomes, participants in the 1st Human Cell Atlas Jamboree, John C. Marioni
The paper introduces a statistical method to distinguish real cells from empty droplets in single-cell RNA sequencing data, improving detection of low-UMI cell libraries while controlling false discovery rates.
Genome biology · Mar 22, 2019Peer-reviewedBioinformatics