An Orthogonal T7 Replisome for Continuous Hypermutation and Accelerated Evolution in E. coli
Christian S. Diercks, Philipp Sondermann, Cynthia Rong, David A. Dik, Thomas G. Gillis, Yahui Ban, Peter G. Schultz
The paper describes an orthogonal T7 replisome system in E. coli that enables continuous hypermutation of genes, leading to accelerated evolution of protein functions.
bioRxiv · Jul 25, 2024 · PreprintSynthetic Biology