Highly efficient scarless knock-in of reporter genes into human and mouse pluripotent stem cells via transient antibiotic selection
V. M. Sluch, X. Chamling, C. Wenger, Y. Duan, D. S. Rice, D. J. Zack
This paper introduces a method for efficient scarless gene knock-in into human and mouse pluripotent stem cells using transient antibiotic selection, achieving up to 65% efficiency for single reporter genes.
bioRxiv · Jul 23, 2018Cell Biology