What Can We Count On? Performance of Microplate Cell Counting Assays in 2D Monolayer and 3D ECM-based In Vitro Tumour Models
Mahsa Vaezzadeh, Annemarie Nadort, Alexandra Igrunkova, Vivienne S. Lee, Antonio Di Ieva, Benjamin Heng, Anna Guller
This study evaluates three cell counting assays in 2D and 3D in vitro models, finding that 2D cultures yield more precise and reliable results than 3D ECM-based cultures.
bioRxiv · Apr 30, 2026 · PreprintBioengineering