A new in vitro assay measuring direct interaction of nonsense suppressors with the eukaryotic protein synthesis machinery
M. Y. Ng, H. Zhang, A. Weil, V. Singh, R. M. Jamiolkowski, A. Baradaran-Heravi, M. Roberge, A. Jacobson, W. Friesen, E. Welch, Y. Goldman, B. S. Cooperman
The paper introduces a new in vitro assay to measure direct nonsense suppressor-induced readthrough and identifies two distinct mechanisms among tested suppressors.
bioRxiv · Nov 7, 2018Biochemistry