Promoted Read-through and Mutation Against Pseudouridine-CMC by an Evolved Reverse Transcriptase
Zhiyong He, Weiqi Qiu, Huiqing Zhou
The paper develops a method called Mut-Psi-seq to map pseudouridine at base-resolution using an evolved reverse transcriptase and chemical labeling, revealing new Psi sites in human rRNAs.
Communications Biology · Jan 11, 2025Peer-reviewedBiochemistry