A Cost-Effective CRISPR/Cas9-Based Method for Sequencing the Functional Antibody Kappa Chain cDNA from Hybridomas Expressing Aberrant Kappa Chain mRNAs
Armin Ahnoud, Chong He, Michael G. Strainic, Wenwu Zhai
The paper develops a CRISPR/Cas9-based method to reduce aberrant kappa chain mRNA sequences in hybridoma clones, achieving up to 88% reduction in a test case.
bioRxiv · Jun 8, 2023 · PreprintMolecular Biology