Co-targeting strategy for precise, scarless gene editing with CRISPR/Cas9 and donor ssODNs in Chlamydomonas
Soujanya Akella, Xinrong Ma, Romana Bačová, Zachary P. Harmer, Martina Koláčková, Xiaoxue Wen, David Alan Wright, Martin H. Spalding, Donald P. Weeks, Heriberto D. Cerutti
The paper describes a co-targeting strategy using CRISPR/Cas9 and ssODNs to achieve precise, scarless gene editing in Chlamydomonas without exogenous dsDNA.
PLANT PHYSIOLOGY · Sep 2, 2021Peer-reviewedGenetics