In-planta Gene Targeting in Barley using Cas9, with and without Geminiviral Replicons
T. Lawrenson, A. Hinchliffe, M. Clarke, Y. Morgan, W. Harwood
This study demonstrates successful heritable gene targeting in barley using Cas9 without a replicon, but fails to achieve heritable events with a replicon despite high repair template copy numbers.
bioRxiv · Feb 4, 2021Plant Biology