Ubiquitin Chloromethylketone Probe Enables Activity-based, Selective Protein Profiling of E2 Ubiquitin Conjugating Enzymes
Saibal Chanda, Alan Pham, Yugendar R. Alugubelli, Nathan Axtell, SreeNidhi Karnati, Yaswanth Aravapalli, James X. Mao, Wenyue Cao, Wenshe Ray Liu
This paper introduces UbCMK, a probe that selectively profiles E2 ubiquitin conjugating enzymes and DUBs, revealing their activity under various stress conditions.
Journal of the American Chemical Society · Sep 7, 2026Peer-reviewedBiochemistry