Directed evolution for improved total secretory protein production in Escherichia coli
David González-Pérez, James Ratcliffe, Shu Khan Tan, Mary Chen May Wong, Yi Pei Yee, Natsai Nyabadza, Jian‐He Xu, Tuck Seng Wong, Kang Lan Tee
This study uses directed evolution to enhance the secretory protein production capacity of OsmY in E. coli, resulting in a significant increase in secreted protein yield.
bioRxiv · Nov 3, 2020 · PreprintSynthetic Biology