AI-driven discovery of host thioredoxin as a CRISPR enhancer of phage-encoded miniature Cas12 hacker nuclease
Zhipeng Wang, Yujue Wang, Hui Gao, Jian Dai, Na Tang, Yannan Wang, Quanjiang Ji
This study identifies thioredoxin as a host factor that enhances the DNA cleavage efficiency of a phage-encoded Cas12 nuclease using AI-driven analysis and biochemical validation.
bioRxiv · Jan 20, 2025 · PreprintMicrobiology