PBMC Fixation and Processing for Chromium Single-Cell RNA Sequencing
Jinguo Chen, Foo Cheung, Rongye Shi, Huizhi Zhou, Wenrui Lu, Julián Candia, Yuri Kotliarov, Katie R. Stagliano, John S. Tsang
This paper introduces a methanol fixation method for preserving PBMCs for scRNA-seq, showing it maintains RNA integrity and transcriptional profiles comparable to live cells.
Journal of Translational Medicine · Jul 17, 2018Peer-reviewedGenomics