A split GFP system to enhance spatial and temporal sensitivity of translating ribosome affinity purification (TRAP)
Kasia Dinkeloo, Zoe Pelly, John M. McDowell, Guillaume Pilot
This paper introduces a modified TRAP system using split GFP to enhance sensitivity and temporal resolution for isolating translatomes from cells with weak promoter activity or low ribosome turnover.
The Plant Journal · Apr 18, 2022Peer-reviewedMolecular Biology