Fluorescently guided workflow with rationally engineered 5' ligation adapters for high-sensitivity and low-bias small RNA sequencing
Sarah Anne Barnes, Daniel Lovíšek, Natalia Dzurcaninova, Martin Čarnecký, Stanislava Bírová, Ivana Čirková, Ján Matyašovský, Adrián Szobi, Pavol Čekan
This paper introduces a new small RNA sequencing workflow that reduces bias and increases sensitivity by using rationally designed adapters and fluorescence-guided band excision.
bioRxiv · Jun 24, 2026 · PreprintMolecular Biology