Long-range PCR amplification and nanopore sequencing of 8-10 kb mitochondrial fragments from environmental DNA
Stephanie A. Matthews, Olivia M. Scott, Yin Cheong Aden Ip, Elizabeth Andruszkiewicz Allan, Ryan P. Kelly
The paper demonstrates the use of long-range PCR and nanopore sequencing to obtain and analyze long mitochondrial fragments from environmental DNA samples, enabling species identification despite sequencing errors.
bioRxiv · Jan 14, 2026 · PreprintBioinformatics