Non-enzymatic isothermal strand displacement and amplification (NISDA) does not enable sensitive nucleic acid quantification.
Thijs Van der Snickt, Scott Ailliet, Simone Bassini, Anouk Peymen, Ahmet Colaker, Enrico Cadoni, Alejandro Valverde, Elise Daems, Annemieke Madder, Karolien De Wael, Pieter Mestdagh
This study attempts to replicate the claimed sensitivity of NISDA but fails to achieve detectable signals except at very high concentrations.
bioRxiv · May 19, 2025 · PreprintMolecular Biology