OPTIMIZATION OF DRIED BLOOD SPOT ASSAYS FOR HEPATITIS B VIRUS SURFACE ANTIBODY QUANTIFICATION
Patience C. Motshosi, Bonolo Bonita Phinius, Mosimanegape Jongman, Kabo Baruti, Lynnette N. Bhebhe, Graceful Mulenga, Wonderful Tatenda Choga, Sikhulile M. Moyo, Simani Gaseitsiwe, Motswedi Anderson
The paper optimizes a dried blood spot (DBS) elution protocol for hepatitis B surface antibody (anti-HBs) quantification, finding that 450 or 500uL PBS and 3hr agitation yield results comparable to plasma, with 25°C storage being optimal.
PLoS ONE · May 27, 2025Peer-reviewedMicrobiology