Optimised biomolecular extraction for metagenomic analysis of microbial biofilms from high-mountain streams
Susheel Bhanu Busi, Paraskevi V. Pramateftaki, Jade Brandani, Stilianos Fodelianakis, Hannes Peter, Rashi Halder, Paul Wilmes, Tom J. Battin
The paper benchmarks nucleic acid extraction protocols for microbial biofilms in glacier-fed streams, finding an adapted phenol-chloroform method yields better DNA quality and taxonomic profiles than commercial methods.
PeerJ · Oct 27, 2020Peer-reviewedMolecular Biology