Deep Learning-Based 3D Leukocyte Differentiation Using Label-Free Higher Harmonic Generation Microscopy
Mengyao Zhou, Patrick José González, Tamara Dekker, Shiqi Zhang, Leonoor S. Boers, Helene B. van den Heuvel, Annemiek Dijkhuis, Iris A. Simons, JanWillem Duitman, Marie Louise Groot
This paper develops a deep learning framework for automated 3D leukocyte differentiation using label-free HHGM, achieving >96% accuracy and comparable results to manual cytospin analysis.
Journal of Translational Medicine · May 12, 2026Peer-reviewedBiophysics