Programmable downsizing of CRISPR-Cas9 activity for precise and safe genome editing
Masaki Kawamata, Hiroshi I. Suzuki, Ryota Kimura, Atsushi Suzuki
The paper introduces a method to programmably reduce CRISPR-Cas9 activity by modifying sgRNAs, enabling precise and safer genome editing with reduced off-target effects and improved mono-allelic editing.
bioRxiv · Oct 31, 2020 · PreprintMolecular Biology