Absolute Membrane Potential Recording with ASAP-Type Genetically Encoded Voltage Indicators Using Fluorescence Lifetime Imaging
Anagha Gopalakrishnan Nair, Marko Rodewald, Hyeonsoo Bae, Philipp Rühl, Jürgen Popp, Michael P. Schmitt, Tobias Meyer‐Zedler, Stefan H. Heinemann
This paper evaluates genetically encoded voltage indicators using FLIM to measure absolute membrane potential, finding that rEstus-NI provides the highest sensitivity and accuracy.
ACS Chemical Neuroscience · Nov 22, 2025Peer-reviewedPhysiology