Author

Jacob E. Greene

University of WashingtonORCID 0000-0002-3016-7296Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Assay:ChIP-seq· 2 Research Focus:differential expression· 2 Approach:experimental assay· 2 Research Focus:gene regulatory network inference· 2 Organism:human· 2 Assay:ATAC-seq· 1 Research Focus:cell type annotation· 1 Assay:CUT&Tag· 1

Frequent Co-Authors

Kami AhmadiD· 2 shared Steven HenikoffiD· 2 shared Weifang WuiD· 1 shared

Activity by Year

2026-2026 · peak 2/yr

Co-Author Collaboration Network

3 top collaborators · 3 mutual co-author links

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2 shared papersGenomics

Kami AhmadORCID

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2 papers

Recent·Most cited

KAS-CUT&Tag for direct mapping of transcription bubbles

Weifang Wu, Jacob E. Greene, Kami Ahmad, Steven Henikoff

N3-kethoxal labeling of single-stranded DNA within CUT&Tag maps Pol II transcription bubbles; bubble density tracks nascent transcription, shifts with transcription inhibitors and TFIIS loss, and peaks at histone genes.

bioRxiv · Oct 9, 2026 · PreprintGenomics✦ In-depth digest

Histone H3K27 methylation states are sequentially catalyzed in cycling cells

Jacob E. Greene, Kami Ahmad, Steven Henikoff

The paper tracks H3K27 methylation dynamics in human cells using CUT&Tag, showing that tri-methylation is maintained stepwise after DNA replication and di-methylation occurs at inactive genes post-replication.

bioRxiv · May 4, 2026 · PreprintGenomics