Chaohai Wei

The paper develops a method to annotate genomic dark matter in Ca. Accumulibacter by integrating pangenome analysis, protein structure prediction, and metatranscriptomics, significantly improving gene annotation coverage.

The paper uses integrated genomic analyses to show that horizontal gene transfer of phoU and ppk2 genes led to Pho dysregulation, enabling phosphorus cycling in Ca. Accumulibacter.

This study systematically analyzes CRISPR-Cas systems and prophages in Candidatus Accumulibacter, revealing their role in phage resistance and adaptation across different taxa and environments.