Guanglei Qiu

The paper develops a method to annotate genomic dark matter in Ca. Accumulibacter by integrating pangenome analysis, protein structure prediction, and metatranscriptomics, significantly improving gene annotation coverage.

The paper uses integrated genomic analyses to show that horizontal gene transfer of phoU and ppk2 genes led to Pho dysregulation, enabling phosphorus cycling in Ca. Accumulibacter.

This study systematically analyzes CRISPR-Cas systems and prophages in Candidatus Accumulibacter, revealing their role in phage resistance and adaptation across different taxa and environments.

The paper describes methods to validate long read assembled genomes using short read metagenome assembled genomes and assesses correction procedures' impact on genome quality, contributing new closed genomes from wastewater bioprocesses.

The paper demonstrates obtaining whole bacterial chromosomes from metagenomic samples using long-read sequencing and presents a pipeline with frame-shift correction.

The paper evaluates extraction solvents for untargeted metabolomics analysis of EBPR bioreactors, finding that optimal methods vary by reactor type.