A rapid, automation-friendly, cell-free and purification-free pipeline for binding and kinetic characterisation of de novo designed binders with high quality standardised per-design data
Diego Ruiz Espi, Gustavo Avelar Molina, Oliver Sonne Buch, Julia Heyd, Andreas Unger, Line W Gormsen, Katrine Boldsen Lund, Kritika Sahni Ray, C. B. Williams, Andreas Hald, Timothy P. Jenkins, Esperanza Rivera‐de‐Torre
This paper presents a rapid, automation-friendly pipeline for characterizing de novo protein binders without cell culture or purification, achieving binding affinity and kinetics in three days.
bioRxiv · Oct 20, 2026 · PreprintMolecular Biology