Calibrating for absolute microbiome abundances without spike-ins
Nimrod Theodoor de Wit, Amulya Baral, Alessandro Fuschi, Guusje Jacobs, Sharona de Rijk, Rozemarijn Q. J. van der Plaats, Ágnes Becsei, Jesse J. Kerkvliet, Rebecca Freitag, Martina Vojtkova, Christian Brinch, Heike Schmitt, Patrick Munk
This paper introduces MGCalibrator, a tool for spike-in free absolute abundance estimation in metagenomics, validated against qPCR and applied to track antibiotic resistance genes in wastewater.
bioRxiv · Jun 24, 2026 · PreprintMicrobiology