Daisuke Tsugama

Genotype imputation improves GWAS resolution and facilitates candidate gene identification in pearl millet

The paper improves GWAS resolution in pearl millet by imputing missing genotypes using a whole-genome sequencing reference panel, leading to more localized association signals and candidate gene identification.

Grass Expression Atlas: an RNA-seq-based expression resource for grass species.

Grass Expression Atlas provides an RNA-seq-based resource for gene expression across diverse grass species and conditions.

DEAR-OWL: a fully browser-based hybrid resource for instant or precise differential gene expression analysis

DEAR-OWL provides a browser-based tool for differential gene expression analysis using RNA-seq data, offering both fast screening and rigorous statistical validation without server dependencies.

Decoding universal principles of codon-mediated regulation of gene expression

The paper develops a regression-based model to predict mRNA and protein abundance using codon weights derived from transcriptomic data, validated across multiple species.

Generation of promoters enabling high-level constitutive gene expression in both plants and Escherichia coli

The paper develops a dual-host promoter system that enables high-level constitutive gene expression in both plants and E. coli, simplifying genetic component validation.

Rapid and flexible assessment of gene functions in plant cells with particle bombardment and linear DNA

The paper improves a low-cost biolistic system for plant gene function analysis by reducing tissue damage, enabling rapid expression, and introducing a sensitive dual-luciferase assay.

A tool to shoot genes with massive air from a compressor (TSGMAC)

The paper develops a cost-effective, helium-free particle bombardment system for plant transformation using a compressor and air duster gun, achieving stable transgenic plants in rice calli.