Stefano Mariani

Refining a molecular tool kit to capture tropicalization in Mediterranean Marine Protected Areas

The paper detects non-indigenous species in Mediterranean marine protected areas using eDNA and UVC, finding that aqueous eDNA metabarcoding provides the most comprehensive species list.

Opportunistic diver-assisted eDNA sampling unpicks fine-scale ecological and conservation signals in tropical reef fishes

The paper uses eDNA collected by divers to assess fish biodiversity and ecological patterns in Seychelles reefs, finding higher diversity in protected and coral areas.

Recovering historical fish eDNA from museum-preserved Antarctic filter feeders via non-destructive metabarcoding.

This study evaluates non-destructive eDNA extraction from ethanol preservatives in Antarctic filter-feeding museum specimens, finding that ethanol filtration performs as well as tissue biopsies for metabarcoding fish eDNA.

DNA Divers: volunteer-based eDNA capture for local and global marine biodiversity monitoring

This paper evaluates a volunteer-based eDNA capture method using swimming motion to collect marine biodiversity data, finding it effective for detecting diverse species across wide latitudinal ranges.

Spatial and seasonal biodiversity variation in a large Mediterranean lagoon using environmental DNA metabarcoding through sponge tissue collection

The paper assesses faunal composition and its spatial and temporal variation in a Mediterranean lagoon using eDNA metabarcoding from sponge tissues collected in different seasons and areas.

Complementary assessment of fish biodiversity across the upper/lower mesophotic interface in a subtropical coral reef using eDNA metabarcoding and baited cameras

This study assesses fish biodiversity at the upper/lower mesophotic interface using eDNA metabarcoding and baited cameras, finding complementary species detections and continuity with shallow reef systems.

Unlocking Antarctic molecular time-capsules - recovering historical environmental DNA from museum-preserved sponges

The paper recovers historical environmental DNA (heDNA) from Antarctic sponge specimens to reconstruct past fish biodiversity and community composition.

Universal closed-tube barcoding for monitoring the shark and ray trade in megadiverse conservation hotspots

The paper develops a portable DNA barcoding method using deep learning to identify shark and ray species in trade, achieving 79.41% accuracy for 28 species.

Shark-dust: High-throughput DNA sequencing of processing residues unveils widespread trade in threatened sharks and rays

Shark-dust DNA sequencing reveals widespread trade in threatened sharks and rays, identifying 54 taxa including many CITES-listed species.

Optimised DNA isolation from marine sponges for natural sampler DNA (nsDNA) metabarcoding

This study optimises DNA isolation from marine sponges for metabarcoding, finding that blotting tissue dry and using a non-commercial protocol improves taxon recovery and sequence reads.

Influence of Preservation Methods, Sample Medium and Sampling Time on eDNA Recovery in a Neotropical River

This study evaluates how preservation methods, sample medium, and sampling time affect eDNA recovery for fish detection in a Neotropical river, finding that cooling water samples outperforms surfactant preservation.