Minming Wang

The paper uses single-cell RNA sequencing to analyze mutant phenotypes of epigenetic regulators in mouse embryos, revealing how these regulators partition the genome based on developmental and evolutionary constraints.

Prime-editing lineage trees for 16 mouse embryos (E7.5 to E10.0, >1.4 million cells) quantify fate restriction timing, progenitor numbers and lineage relationships, and are reproducible across replicate embryos.

This study uses a deep-learning model to analyze scRNA-seq data and identifies how folate deficiency disrupts metabolic transitions in developing mouse neural ectoderm, leading to morphological defects.