An Engineered T7 RNA Polymerase for efficient co-transcriptional capping with reduced dsRNA byproducts in mRNA synthesis
Mathew G. Miller, Oscar Alvizo, Scott Baskerville, Avinash Chintala, Chinping Chng, Justin P. Dassie, Jonathan D. Dorigatti, Gjalt W. Huisman, Stephan Jenne, Supriya Kadam, Neil Leatherbury, Stefan Lutz, Melissa Mayo, Arpan Mukherjee, Antoinette Sero, , Xiyun Zhang
This paper describes an engineered T7 RNA polymerase, T7-68, that improves mRNA synthesis by efficiently co-transcriptional capping and reducing dsRNA byproducts.
Faraday Discussions · Jan 1, 2024Peer-reviewedMolecular Biology