BiophysicsbioRxivPreprint
Oblique Thick Light-Sheet Microscopy with Spatiotemporal 3D Localization for RNA Imaging in Intact 50 μm-Thick Sections
Lin Cai, Qihang Song, Dongjian Cao…·Zhen‐Li Huang
This paper introduces a microscopy technique that enables 3D RNA imaging in thick brain sections by combining spatiotemporal localization with volumetric excitation, revealing detailed neuronal distributions and expression gradients.