Single-step generation of homozygous knock-out/knock-in individuals in an extremotolerant parthenogenetic tardigrade using DIPA-CRISPR
Koyuki Kondo, Akihiro Tanaka, Takekazu Kunieda
This study develops a method to generate homozygous gene-edited tardigrades using DIPA-CRISPR, enabling in vivo analysis of extreme stress tolerance mechanisms.
PLoS Genetics · Jun 13, 2024Peer-reviewedGenetics