Establishment of an efficient transformation and CRISPR/Cas9-mediated gene editing system in Chinese local planting cassava (Manihot esculenta Crantz) cultivar SC8
Yajie Wang, Xiaohua Lü, Xing-Hou Zhen, Hui Yang, Yannian Che, Jing-Yi Hou, Ruimei Li, Jiao Liu, Meng-Ting Geng, Xinwen Hu, Yuan Yao, Jianchun Guo
The paper establishes an efficient Agrobacterium-mediated transformation system and CRISPR/Cas9 gene editing in cassava SC8, achieving high transgenic line production and homozygous mutations.
bioRxiv · Mar 17, 2022 · PreprintMolecular Biology