Endogenous Promotor-Driven Split Nanoluciferase Biosensor for Assessing G Protein Recruitment
Laura J. Humphrys, Carina Höring, Albert Owusu Gattor
The paper uses CRISPR/Cas9 to tag native β-adrenoceptors and a minimal Gs protein with split Nanoluciferase under endogenous promoters in HEK293T cells, enabling selective-ligand characterization without overexpression.
bioRxiv · Jun 3, 2024 · PreprintCell Biology