OTUs clustering should be avoided for defining oral microbiome
Alba Regueira‐Iglesias, Lara Vázquez-González, Carlos Balsa‐Castro, Triana Blanco‐Pintos, Vı́ctor M. Arce, Marı́a J. Carreira, Inmaculada Tomás
This study evaluates primer pairs for 16S rRNA gene amplicon sequencing in the oral microbiome, showing that OTUs defined by 97% similarity often miscluster species, affecting diversity metrics and health-disease associations.
bioRxiv · Aug 9, 2021 · PreprintBioinformatics