BioinformaticsbioRxivPreprint
OTUs clustering should be avoided for defining oral microbiome
Alba Regueira‐Iglesias, Lara Vázquez-González, Carlos Balsa‐Castro…·Inmaculada Tomás
This study evaluates primer pairs for 16S rRNA gene amplicon sequencing in the oral microbiome, showing that OTUs defined by 97% similarity often miscluster species, affecting diversity metrics and health-disease associations.
1 citation