Nested PCR to optimize rpoB metabarcoding for low-concentration and host-associated bacterial DNA
Laëtitia Leclerc, Gabryelle Agoutin, Thierry Brévault, Antony Champion, Jean Mainguy, Nicolas N Negre, Sudeeptha Yainna, Géraldine Pascal, Sophie Gaudriault, Jean-Claude Ogier
This paper introduces a nested PCR approach to optimize rpoB metabarcoding for low-concentration and host-associated bacterial DNA, showing improved amplification efficiency without bias.
Microbiology Spectrum · Aug 12, 2025Peer-reviewedMicrobiology