What are the most influencing factors in reconstructing a reliable transcriptome assembly?
Noushin Ghaffari, Jordi Abante, Raminder Singh, Philip D. Blood, Lenore Pipes, Christopher E. Mason, Charles D. Johnson
The paper investigates factors affecting transcriptome assembly quality, finding that input RNA-Seq data quality and base-calling accuracy are more critical than coverage.
bioRxiv · Nov 16, 2017 · PreprintBioinformatics