Author

Varsha Rao

ORCID 0000-0002-7348-526XUnclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Assay:DNA methylation· 1 Approach:experimental assay· 1 Research Focus:variant calling· 1

Frequent Co-Authors

Andrew A. Mishin· 1 shared Christopher J. TrolliD· 1 shared Ruby Tibet· 1 shared Tobin GrothiD· 1 shared

Activity by Year

2026-2026 · peak 1/yr

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4 top collaborators · 6 mutual co-author links

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1 shared paperGenomics

Andrew A. Mishin

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1 paper

Recent·Most cited

End-repair causes methylation underestimation in cell-free DNA sequencing libraries

Tobin Groth, Andrew A. Mishin, Varsha Rao, Ruby Tibet, Christopher J. Troll

The paper shows that end-repair in double-stranded DNA libraries reduces CpG methylation estimates and tissue origin accuracy, while trimming read termini mitigates this bias but reduces coverage.

The Journal of Liquid Biopsy · Jan 3, 2026Peer-reviewedGenomics