Synthetic BiologyMolecular Systems BiologyPeer-reviewed
Absolute quantification of translational regulation and burden using combined sequencing approaches
Thomas E. Gorochowski, Irina Chelysheva, Mette Eriksen…·Zoya Ignatova
The paper combines Ribo-seq and RNA-seq to quantify translation initiation rates, termination efficiencies, and frameshifting in E. coli, revealing insights into translational regulation and stress responses.