Clonally selected lines after CRISPR/Cas editing are not isogenic
Arijit Panda, Milovan Šuvakov, Jessica Mariani, Kristen L. Drucker, Yohan Park, Yeongjun Jang, Thomas M. Kollmeyer, Gobinda Sarkar, Taejeong Bae, Ji‐Eun Kim, Wan Hee Yoon, Robert Brian Jenkins, Flora Maria Vaccarino, Alexej S. Abyzov
The paper shows that clonally selected CRISPR-edited cell lines acquire many culture-specific mutations, making them non-isogenic, and recommends comparing multiple edited and unedited line mixes.
The CRISPR Journal · Apr 1, 2023Peer-reviewedGenomics