Comparative framework and adaptation of ACME HS approach to single cell isolation from fresh-frozen endocrine tissues
Marina V. Utkina, Anastasia S. Shcherbakova, Ruslan M. Deviatiiarov, Alina Ryabova, Marina Yu. Loguinova, Valentin Trofimov, Anna A. Kuznetsova, Mikhail Petropavlovskiy, Rustam Kh. Salimkhanov, Denis O. Maksimov, Eugene A Albert, Alexandra Golubeva, Walaa Asaad, Liliya S. Urusova, А. М. Лапшина, , Sergey Popov
This study adapts the ACME HS method to isolate intact single cells from fresh-frozen endocrine tissues, showing it preserves cell integrity and gene expression better than other methods.
Frontiers in Cell and Developmental Biology · Jan 7, 2025Peer-reviewedGenomics