Developmental BiologyPNAS NexusPeer-reviewed
Ablation of OCT4 function in cattle embryos by double electroporation of CRISPR-Cas for DNA and RNA targeting (CRISPR-DART)
Jada L Nix, Gustavo Pimenta Schettini, Savannah L. Speckhart…·Fernando H. Biase
This study improves the efficiency of biallelic deletions in cattle embryos using CRISPR-Cas and shows OCT4 is critical for blastocyst formation and pluripotency.
7 citations