Heat shock-mediated transformation is possible in several Gram-negative bacteria
Shubhra Jyoti Giri, Pankaj Losan Sharma, Lukapriya Dutta, Shuhada Begum, Shuvam Bhuyan, Monika Jain, Niraj Agarwala, Suvendra Kumar Ray
This study demonstrates that heat shock transformation can be successfully applied to four Gram-negative bacteria beyond E. coli, enabling gene introduction and fluorescence expression.
Antonie van Leeuwenhoek · Apr 3, 2026Peer-reviewedMicrobiology