DNA extraction and host depletion methods significantly impact and potentially bias bacterial detection in a biological fluid
Erika Ganda, Kristen L. Beck, Niina Haiminen, Justin D. Silverman, Ban Kawas, Brittany D. Cronk, Renee R. Anderson, Laura B. Goodman, Martin Wiedmann
The paper evaluates DNA extraction and host depletion methods in bovine milk to improve bacterial detection for food safety, finding that magnetic-based methods are most effective and some methods introduce microbial detection bias.
mSystems · Jun 15, 2021Peer-reviewedMolecular Biology