Pepper RNA variants reveal decoupling of HBC530 binding thermodynamics and fluorescence activation
Emmanuel K. Aidoo, Vishakha Jayasekera, Nkosinathi Dlamini, Patrick H. Dussault, Catherine D. Eichhorn
The paper evaluates 53 Pepper RNA variants to show that HBC530 binding affinity and fluorescence intensity are only modestly correlated, with distal structural elements influencing both and Mg2+ binding being strongly correlated with HBC530 binding thermodynamics.
bioRxiv · Jun 24, 2025 · PreprintBiophysics