GenomicsbioRxivPreprint
Topology-aware thermodynamics for DNA probe design under fixed stringency: Retained paired boxes link mismatch placement, nearest-neighbor stability and room-temperature diagnostic specificity
Ivan B. Brukner, Maja Krajinović
The paper introduces a topology-aware thermodynamics framework for DNA probe design, showing that mismatch placement and retained paired boxes significantly affect specificity and signal ratios.
DOI 10.64898/2026.06.10.731169