BioinformaticsbioRxivPreprint
Beyond Bisulfite Sequencing: Resolving 5-hmC with Nanopore Sequencing Unmasks the True-5mC Methylation Entropy Landscape
Uri Bertocchi, Eyal Katz, Jonathan Jeffet…·Yuval Ebenstein
The paper compares True-5mC methylation entropy analysis using nanopore sequencing to bisulfite sequencing in kidney cancer and mouse brain, showing bisulfite introduces systematic biases affecting biological interpretation.