Synthetic BiologyPlant Biotechnology JournalPeer-reviewed
Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomato
Tien Van Vu, Velu Sivankalyani, Eun‐Jung Kim…·Jae‐Yean Kim
This study improves HDR-based genome editing efficiency in tomato using CRISPR/LbCpf1 multi-replicon systems, achieving stable HDR alleles without antibiotic markers or phenotypic selection.
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